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Make a choice dr. P.K. Sharma H.C. van der Veen

Project properties

Title	Influence of particle size in minced autologous cartilage implantation
Keywords	regeneration cartilage joint
Researchers	dr. P.K. Sharma H.C. van der Veen
Type of project	Pilot project (year 2 or 3), Stage Wetenschap / Researchproject of MD/PhD programme
Nature of the research	Experimental
Fields of study	biomaterials orthopedics

Background / introduction
Cartilage defects predispose to development of osteoarthritis especially in the knee joint. Mincing cartilage is a promising technique in treating these defects.

Research question / problem definition
Does the particle size influence the ingrowth capacity of minced cartilage in an in vitro cartilage defect model

Workplan

Bovine cartilage taken from 2 year old bull knees will be used for all the experiments. Osteochondral plugs will be prepared with and without a circular chondral defect. Cartilage harvested from elsewhere from the same knee joint will be minced at different particle sized and filled in the gap with or without the fibrin glue. The plug will be cultured at 37 degrees and a relevant medium for 14 days and then at 1, 7 and 14 days the cartilage will be inspected with histology and immunohistochemical staining for cartilage relevant ECM components i.e. collagen and proteoglycans. Gene expression will also be studied if necessary. Yet another variable will be studied where the cartilage will be loaded in a bioreactor both for normal and shear forces. And the effect on cartilage wound healing will be determined.

References

Salzmann GM, Baumann GA, Preiss S. Spontaneous Minced Cartilage Procedure for Unexpectedly Large Femoral Condyle Surface Defect. Case Rep Orthop. 2016;2016:1498135. doi:10.1155/2016/1498135 Salzmann GM, Ossendorff R, Gilat R, Cole BJ. Autologous Minced Cartilage Implantation for Treatment of Chondral and Osteochondral Lesions in the Knee Joint: An Overview. Cartilage. 2021 Dec;13(1_suppl):1124S-1136S. doi: 10.1177/1947603520942952. Epub 2020 Jul 25. PMID: 32715735; PMCID: PMC8808955. Bonasia DE, Marmotti A, Mattia S, Cosentino A, Spolaore S, Governale G, Castoldi F, Rossi R. The Degree of Chondral Fragmentation Affects Extracellular Matrix Production in Cartilage Autograft Implantation: An In Vitro Study. Arthroscopy. 2015 Dec;31(12):2335-41. doi: 10.1016/j.arthro.2015.06.025. Epub 2015 Aug 28. PMID: 26321111.