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allergology (9)
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epidemiology and statistics (20)
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haematology (25)
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health sciences (22)
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intensive care (28)
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laboratory medicine (8)
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medical education (6)
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microbiology (23)
molecular biology (34)
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movement sciences (15)
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obstetrics (4)
oncology (77)
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Patient Related Research (10)
pediatrics (83)
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Stamcelbiologie (3)
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surgery (94)
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urology (10)
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(Epi)genetic epidemiology (1)
(medical)genetics (5)
5jaars overleving (1)
:psychosocial and lifestyle determinants (4)
aanhoudende lichamelijke klachten (ALK) (1)
abdomen (1)
access to medicine (1)
ACE-inhibitor (1)
Acetabular fracture (1)
Acetylcholine (1)
Achilles tendinopathy (1)
Achilles tendinopatie (1)
ACS-NSQIP surgical risk calculator (1)
activated clotting time (1)
Acute flaccid myelitis (1)
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Acute Myeloid Leukemia (1)
acute myocardial infarction (2)
adpkd (3)
Advanced cell culture (1)
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adverse side effects (1)
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ageing (4)
ageing lungs (1)
Ageing, Nuclear Envelope, Nuclear Pore Complex (1)
aging (1)
airway epithelium (1)
Alcohol (3)
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Allergic asthma (1)
Allergologie (1)
Allergy (1)
allogene stam cel transplantatie (2)
Allogeneic stem cell transplantation (1)
Amalgaam (2)
ambulance (1)
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ANCA associated vasculitis (1)
anesthesia (5)
Anesthesiologie (1)
anesthesiology (2)
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Animal research (2)
ankylosing spondylitis (2)
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antibiotics (3)
anticoagulants (2)
antidepressants (4)
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anxiety (1)
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aortic stenosis (1)
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Appendicitis (2)
arrhythmia (1)
arterioveneuze fistel, dialyse shunt (1)
Artificial Intelligence (3)
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asthma (7)
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astma (1)
asylum seekers (1)
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atherosclerosis (3)
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autoimmune blistering diseases (1)
autoimmune disease (6)
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Autophagy (2)
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beta cell destruction (1)
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biofilm (1)
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brain (3)
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brain tumour (2)
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Breast augmentation mammoplasty (2)
breast cancer (5)
breast conserving surgery (1)
Breast implants (1)
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bronchial epithelium (1)
built environment (1)
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Calorie restriction (1)
Calprotectin (2)
cancer (30)
cancer cell apoptosis (1)
Cancer cell metabolism (1)
cancer cells (1)
cancer patients (2)
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Cardiac Amyloidosis (2)
cardiac differentiation (1)
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cardiac output (1)
cardiac surgery (1)
cardio-pulmonary toxicity (1)
cardiogenetics (2)
cardiology (10)
cardiometabolic health (1)
cardiopulmonary bypass (1)
Cardiovasculaire effecten (1)
cardiovascular (4)
cardiovascular disease (6)
cardiovascular risk (1)
cardiovascular risk management (1)
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caries (1)
carriers (1)
cartilage (2)
Catheterization lab (1)
celiac disease (4)
cell biology (1)
cell cycle (2)
cell death (3)
cell saver (1)
cellbiology (1)
cellular interactions (1)
Central Sensitization (nociplasticity) (1)
cerebral palsy (1)
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Cervical spine (2)
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child (2)
Child development (1)
child outcome (2)
childbirth (1)
children (9)
ChIP assay (1)
cholangiocyte (1)
cholestasis (1)
cholesterol fluxes (1)
chromosome (1)
chronic depression (2)
chronic disease (2)
Chronic Kidney Disease (5)
chronic lung allograft dysfunction (1)
Chronic rhinosinusitis (1)
chronic ventilatory support (1)
Chronische pijn (2)
Circadian rhythmicity (1)
cirrhosis (2)
Clinical decision making (1)
clinical decision-making (1)
clinical diagnostics (4)
clinical epidemiology (1)
clinical genetics (1)
Clinical retrospective patient outcome study (1)
Clinical Severity Score (1)
co-morbidity (1)
coagulation (1)
coating (1)
coccygodynie (1)
cochlear implant users (1)
Cognition (1)
cognition and outcome (2)
cognitive decline (1)
cognitive function (3)
cognitive impairment (1)
cognitive testing (1)
collect clinical information (1)
Colon carcinoma (1)
colonisation (1)
colorectal cancer (2)
colorectal cancer, CEA value, CT-scan, follow up (1)
colorectal surgery (3)
comorbidity: anxiety, aggression, emotion regulation problems, depression (1)
compensatory movements (1)
competency development (1)
complement (3)
complex congenital heart disease (1)
compliance (1)
complications (4)
Comprehensice geriatric assessment (1)
computed tomography (CT) (3)
Congenital anomalies (1)
congenital heart disease (3)
connective tissue disease (1)
Constipation (1)
continue glucose monitoring (1)
contraction (1)
contrastmiddel (1)
COPD (14)
Coronair angiografie (1)
coronary arteries (1)
coronary artery bypass grafting (1)
Coronary Artery Disease (3)
cosmetic outcome (1)
costs (3)
cough (1)
COVID-19 (6)
craniopharyngioma (2)
Craniotomy (1)
Critically ill children (3)
Crohn's disease (2)
Crohn's disease (3)
Crohn's disease (1)
CT (1)
CT imaging (2)
CT-scan (1)
cystic fibrosis (4)
cytokines (1)
Damage (1)
database (3)
database registry (1)
Deep brain stimulation (DBS) (6)
deletion (2)
delirium (1)
delivery agent (1)
dementia (3)
dental erosion (1)
dental hygiene (1)
dentist (1)
Deprescribing (1)
depression (4)
Depression, (2)
Depression, translational neuroscience (1)
dermatology (3)
Descriptive and retrospective study with clinical results (1)
development (3)
Developmental coordination disorder (1)
developmental disorder (1)
developmental disorders (1)
Developmental Dysplasia of the Hip (DDH) (2)
developmental psychopathology (1)
Dexamethasone (1)
diabetes (3)
Diabetes mellitus (4)
diabetis (1)
diagnostic importance (1)
diagnostic test (1)
diagnostics (3)
dialysis (3)
Diaphragm paralysis (1)
Diet (2)
dilated cardiomyopathy (1)
disease activity (1)
disease models (1)
Disease modifying therapy (1)
DNA (1)
DNA damage (1)
DNA damage response (1)
DNA methylation signatures (1)
donor (1)
Drosophila (2)
drug delivery (1)
Drug Interactions (1)
dry eye (2)
duplication (1)
Dupuytren's disease (1)
Dupuytren's disease (1)
Dystonia (6)
e-health (5)
early detection (1)
early life experiences (1)
Early Onset Ataxia (2)
eating disorders (1)
echo duplex, diagnotische waarde (1)
echocardiography (2)
eco-anxiety (1)
Education (2)
EEG-EMG (1)
effects (1)
Ehealth (10)
Elder (1)
elderly (6)
elderly cancer patients (1)
elderly patients (2)
electroconvulsive therapy (1)
Electronic Nose (1)
EMG (2)
EMG-analysis (1)
emphysema (1)
EMSA (1)
enamel (1)
end-expiratory lung volume (1)
endocrine disrupting chemicals (1)
endocrine therapy (1)
endocrinology (1)
endometriosis (1)
Endophenotypes (1)
Endothelial Cell (2)
endothelial cells (1)
endoveneuze laser behandeling (1)
energy metabolism (3)
engineering (1)
enkelletsel (1)
eNose (1)
epidemiology (9)
epidermolysis bullosa (2)
epigenetics (4)
epithelial cells (1)
epithelial disorders (1)
ercp (1)
errors (1)
erythropoietin (1)
Esophageal cancer (1)
esophageal carcinoma (2)
Esophageal pressure (1)
esophagus surgery (2)
Essential tremor (3)
ESWL (2)
ethics (1)
evidence-based medicine (1)
EVLT (1)
Ex vivo lung perfussion (1)
exercise testing (1)
exercise therapy (1)
Exercise training (1)
Exergames (1)
exome sequencing (3)
Faecalibacterium prausnitzii (1)
familial hypercholesterolemia (1)
Family history (2)
Farmacologie (1)
farmacology (1)
fatigue (4)
fatty acids (1)
fatty liver disease (1)
FDG PET/CT (2)
fecal incontinence (1)
fertility (1)
fetal gender (1)
fibrosis (3)
filling (2)
First Episode Psychosis (2)
first trimester anomaly scan (1)
first trimester ultrasound (1)
fitness (1)
fluid administration, hemodynamic monitoring (1)
fluid management (1)
Fluorescence imaging (1)
fMRI (1)
fNIRS-monitoring (1)
foot ulcers (1)
frailty (3)
Frailty screening (2)
Function Endoscopic Sinus Surgery (1)
functional genetics (2)
functional somatic symptoms (1)
Functional stroke mimic (1)
functionele symptomen (1)
Fungal Infections (1)
fysiotherapie (1)
gait (1)
galectin-3 (1)
Galgangatresie (1)
game (1)
gangliosides (1)
GBA1-gene (1)
GECKO (1)
Gender (1)
gene enrichment (1)
gene expression (1)
gene regulation (1)
general movements (1)
general practice (3)
general practitioner (1)
genetic diseases (1)
Genetic sequencing (1)
genetic testing (1)
genetics (5)
genome analysis (1)
genomic instability (1)
genotype-phenotype correlation (1)
geriatric oncology (1)
geriatrics (2)
glaucoma (2)
Glioblastoma (1)
Gliomas (1)
global child health (1)
global health (2)
Glomerular filtration rate (1)
Glucocorticoids (1)
glucose homeostasis (1)
growth hormone deficiency (1)
Guidelines (2)
Guillain-Barré syndrome (1)
Gunanine Nucleotide Exchange Factor (1)
Gut Microbiota Composition (2)
gut-liver acis (1)
haemophilia (2)
hand (1)
Head and neck cancer (5)
Health care professionals (1)
Health care utilization (1)
health economics (3)
Health indicators (1)
Health literacy (1)
Health Related Quality of Life (1)
health-related quality of life (4)
healthcare education (1)
healthcare transformation (1)
hearing impairment (1)
hearing rehabilitation (1)
heart (14)
heart failure (7)
Heart rate variability (1)
heart transplantation (1)
heart valve replacement (1)
heat shock proteins (1)
Heel prick Screening (1)
hematology, cancer, critical care, outcome (3)
hematopoiesis (5)
hemodynamic monitoring (2)
hemodynamics (1)
hemostasis (2)
heparin induced thrombocytopenia (1)
hepatitis (2)
hepatocellular carcinoma (1)
hereditary cancer (1)
hereditary kidney disease (1)
HFOV (2)
Hibernation (4)
Hidradenitis suppurativa (1)
hip (1)
hip and knee prothesis (2)
Hip artroplasty (1)
Historical controls (1)
HIV (3)
Hodgkin lymphoma (2)
Home mechanical ventilation (3)
Honours education (1)
hormone receptors (1)
HPB surgery (1)
huisarts (1)
Huisartsgeneeskunde (1)
human genetics (1)
human hematopoietic stem cells (3)
Humeral Fractures (1)
hydrochlorothiazide (1)
hydrogen sulfide (1)
hymenoptera venom allergy (1)
hyperglycaemia (1)
Hyperkinetic movement disorders (2)
Hypertensie (1)
hypoglycemia (1)
hypophospatemic rickets (1)
hypoplastic left heart syndrome (1)
hypotension (1)
IBD (4)
IGF-1 (1)
image biomarker (2)
imaging (12)
imaging, biomarkers (1)
Immune checkpoint inhibitors (3)
immune response (1)
immune suppression (1)
Immune-related adverse events (1)
immunity (1)
immunology (10)
immunosuppression (1)
immunotherapy (4)
impact (1)
implant infection (2)
in vitro (1)
in vitro fertilization (1)
Inborn Error of Metabolism (1)
incidence (1)
incontinence (2)
individualised treatment (1)
infant (2)
infant assessment (2)
infant development (3)
Infant Stool Colour Card (1)
infection (3)
Infection and inflammation (1)
Infectious diseases (1)
Infective Endocarditis (1)
inflammation (5)
Inflammatory bowel disease (3)
Inflammatory rheumatic disease (3)
Informatie-uitwisseling (1)
Information technology (1)
Inguinal Hernia Surgery (1)
innovatie van zorg (2)
insulin (1)
insulitis (1)
Integrative care (1)
intellectual disability (1)
Intensive care (1)
interactie (1)
interprofessional education (1)
Interviews (1)
Interviewstudie (1)
intestinal disease (2)
intestinal disorder (1)
intestinal integrity (1)
intestinal transplantation (2)
Intestinal wall permeability (1)
intestine (1)
Intracraniele druk (1)
Intraoperative neurophysiology (1)
IONM (1)
iPSC (1)
iron deficiency (2)
ischemia (3)
ischemia-reperfusion injury (1)
ischemia/reperfusion injury (2)
ischemic stroke (2)
Isolation (1)
isotopes (1)
IVF (3)
jaundice (2)
joint (2)
Ki67 proliferation index (1)
kidney (6)
kidney disease (5)
Kidney Donation (3)
kidney transplantation (13)
kidneydonor (1)
Kindergeneeskunde (1)
kinetic modeling (1)
Klinische onderzoeksstage (1)
knockout mouse (1)
kwaliteit van leven (2)
kwaliteit van zorg (1)
laboratory animals (10)
language development (1)
language screening (1)
laparoscopy (1)
laryngectomy (3)
late effects of treatment (1)
Leadership (1)
learning curve sonologist (1)
learning organization (1)
leefstijl (1)
leukemia (3)
leukemic stem cells (2)
LifeLines (2)
lifestyle (4)
liposomes (1)
lipotoxicity (1)
liver (3)
liver cancer (1)
liver disease (5)
liver diseases (1)
liver failure (1)
liver fibrosis (1)
liver metastases (1)
liver resection (1)
liver transplantation (10)
livercirrhosis (1)
locally advanced rectal caner (1)
locomotion (1)
long-term survival (1)
longitudinal (1)
lumg transplantation (1)
lung (4)
lung cancer (5)
Lung function (1)
lung mechanics (2)
lung nodules (2)
lung recruitment (1)
lung transplantation (5)
lymphoma (2)
lynch syndrome (1)
machine learning (Matlab/Rstudio) (1)
machine perfusion (2)
major depressive disorder (1)
mass spectrometry (2)
mast cells (1)
mastocytosis (3)
measurement tool (1)
mechanical ventilation (10)
medical comorbidity (1)
medical device (4)
medical education (2)
medical imaging (8)
medically unexplained symptoms (2)
medication (3)
Medication reviews (1)
medication safety (1)
medication treatment (1)
medicine (1)
melanoma (5)
Meningioma (2)
meno-pauze (1)
mental retardation (1)
metabolic diseases (5)
Metabolic signaling (1)
metabolic syndrome (3)
metabolism (3)
metabolites (1)
metabool syndroom (3)
metformin (1)
method development (2)
microbiome (1)
microbiota (3)
microcephaly (1)
Microglia (1)
microRNA (2)
Minimally invasive parathyroidectomy (1)
mitochondria (1)
MitraClip (1)
Mobile electrocardiogram devices (1)
Mobility (1)
molecular biology (2)
molecular chaperones (1)
Molecular Fluorescence Guided Surgery (1)
molecular mechanisms (1)
molecular microbiology (1)
Molecular tumour board (1)
molecular typing (1)
Monoclonal antibodies (1)
monocytes (1)
mood disorders (1)
Motor development (2)
Mouse models (1)
movement disorders (3)
MRI (8)
MRI / CT (1)
MRI / magnetic resonance imaging (3)
MRI brain (1)
MRS (1)
mucosal melanoma (1)
mucus clearance (1)
multi-morbidity (1)
Multi-organ failure (1)
Multi-phase CT (1)
multidrug resistance (1)
Multimorbidity (3)
multiple myeloma (2)
multiple sclerosis (4)
Musculoskeletal pain (1)
Music (1)
music intervention (1)
music therapy (4)
mutations (2)
myelin (1)
myocardial infarction (2)
myoclonus (1)
myoclonus dystonia (1)
NASH (1)
nasopharyngeal carcinoma (1)
Necrotische Enterocolitis (1)
necrotising enterocolitis (1)
nefropathie (1)
neglected diseases (1)
Neglected Tropical Diseases (1)
neoadjuvant chemoradiotherapy (1)
neonatal hypoglycemia (1)
Neonatal hypoxic-ischaemic encephalopathy (1)
Neonatal outcome (1)
neonatale icterus (1)
neonates (2)
neonatology (1)
nephrology (2)
neuro-endocrine tumors (1)
neurobiology (1)
neurocognition (1)
neurodegeneration (4)
neurodegenerative diseases (3)
neurodevelopmental impairment (1)
neurodevelopmental outcome (1)
neuroendocrine tumor (1)
Neuroimaging (5)
neuroinflammation (1)
neurological development (1)
neurology (1)
neuromuscular blockade (1)
neuropathic pain (1)
neuropathy (1)
neuropsychiatric symptoms (1)
Neurosurgery (4)
neurotransmitters (1)
neutropenia (1)
new genes (1)
nier (1)
non-Hodgkin's disease (1)
Non-invasive ventilation (2)
Non-motor symptoms (2)
Noordzeeziekte (1)
Nuclear Medicine (1)
nuclear receptors (1)
nursing home (1)
Nursing home residentss (1)
nutrient addiction (1)
nutrition (8)
obesity (6)
Occupational exposures (1)
Occupational health (1)
Older adults (1)
oligodendrocytes (1)
oncological epidemiology (1)
oncology (6)
oncoplastic surgery (1)
ongeplande heropname (1)
online wearables (1)
opthalmologic problems (1)
organ preservation (3)
organ transplant (1)
organ transplantation (2)
orthopedics (3)
osteomyelitis (1)
otolaryngology (1)
Ouderen (1)
outcome (2)
outcome and cognition (1)
Outcome Assessment (1)
Outcome measurement (4)
Outcomes (2)
Outocome (1)
ovarian cancer (1)
Overactieve blaas (2)
overweight (2)
Overweight/obesity (1)
oxidative stress (2)
oxygenation (1)
pain (2)
Pain (Chronic) (1)
PALB2 mutation (1)
palliatief (1)
Palliatieve zorg (3)
palliative care (1)
pancreatic resection (1)
pancreatoduodenectomie (1)
paralysis (1)
parental support (1)
Parkinson disease (4)
Parkinson's disease (4)
Parkinson's disease (2)
Parkinson’s disease (1)
Parkinson's disease (4)
Participation restriction (1)
pathological complete response (1)
pathology (1)
pathway analysis (1)
Patiënt-gerichtheid (1)
patient centered care (1)
patient experiences (2)
Patient perspective (1)
patient related research (1)
patient satisfaction (1)
Patient-Reported-Outcomes (1)
PD-L1 immunohistochemistry (1)
pediatric cardiology (2)
Pediatric gastroenterology (1)
Pediatric rehabilitation (1)
pediatrics (6)
Percutane Nefro-Litholapaxy (2)
Percutaneous coronary intervention (PCI) (1)
Percutaneous intervention (2)
Peri-Acetabular Osteotomy (PAO) or Bernese/Ganz osteotomy (2)
perinatal care and psychiatry (1)
perineal wound healing (1)
periodontitis (1)
perioperative care (1)
Periprosthetic joint infection (1)
peritonitis (1)
Persistent somatic symptoms (1)
personal medicine (1)
personalized (1)
Personalized medicine (3)
personalized treatment (1)
PET (6)
pet scintigraphy (1)
PET-tracer (1)
Peyronie disease (1)
pharmacodynamics (1)
pharmacokinetics (1)
pharmacology (4)
Phelan-McDermid syndrome (1)
phenomenology (1)
phenotype-genotype study (1)
phenylketonuria (1)
photometry (1)
physical activity (3)
physical and mental functioning (1)
physical therapy (1)
physiology (2)
placenta (1)
plasmacytoma (1)
plastic surgery (1)
platelet (1)
polisomnography (1)
Polycomb (1)
polypharmacy (7)
population cohort study (1)
population screening (2)
portal hypertension (1)
Positron Emission Tomography (1)
Postgraduate Medical Education (2)
postoperative complications (2)
postoperative outcome (1)
postoperative pain (1)
posttraumatic (1)
postural control (1)
potassium (1)
pre-clinical imaging (1)
Precision medicine (1)
predictiemodel (1)
Predictiemodellen (1)
prediction (1)
prediction modeling (2)
pregnancy (8)
Prehabilitation (1)
Prematuren (1)
prenatal diagnosis (1)
prenatal diagnostics (2)
prenatal programming (1)
preservation fluids (1)
preterm (1)
preterm birth (1)
Prevalence of cancer (1)
prevention (3)
preventive cardiovascular medication (1)
primairy health care (1)
primary care (7)
primary care, gastro-enteritis, children, out-of-hours service, pediatrics. (2)
Primary Hyperparathyroidism (1)
probiotics (1)
Proefschrift (1)
prognosis (3)
Prolapse surgery (1)
proliferation (1)
Promoveren (1)
prospective cohort study (1)
prostate cancer (1)
Prosthetic Valve (1)
protein aggregation (1)
protein kinase inhibitors (1)
protein restoration therapy (1)
proteomics (3)
prothrombin complex concentrate (1)
PSMA PET/CT (1)
Psychiatric symptoms (1)
psychiatry (1)
psychiatry, high-field MRI (1)
psychological stress (1)
Psychology (1)
psychosis (1)
psychosocial (2)
psychosocial and lifestyle determinants (1)
psychotherapy (1)
Publiceren (1)
pulmonary embolism (1)
pulmonary hypertension (2)
pulmonary rehabilitaion (1)
Pulmonary rehabilitation (1)
QST (1)
qualitative research (3)
quality improvement (1)
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Establishing the predictive value of FDG PET/CT for response to ICI in metastatic melanoma and non-small cell lung cancer
Keywords: Immune checkpoint inhibitors
FDG PET/CT
Immune-related adverse events
Researchers:
Dr. T.J.N. Hiltermann
F. Bensch
L.B.M. Hijmering
Nature of the research:
Retrospective data collection of patients treated with immune checkpoint inhibitors for metastatic melanoma and NSCLC
Fields of study:
oncology
Background / introduction
In the last decade the treatment of many cancers has changed fundamentally due to the introduction of immune checkpoint inhibitors (ICI). The addition of cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed death-ligand 1 (PD-L1) checkpoint inhibitors to standard of care in metastatic melanoma and NSCLC has led to substantial improvement of overall survival including earlier unseen durable responses. Initially, PD-L1 status assessed on a tumor biopsy was thought to be a good marker for patient selection. However, multiple studies reported responses also in PD-L1 negative patients, as well as failure of treatment in a substantial proportion of PD-L1 positive patients. Adequate biomarkers to preselect patients for immune checkpoint inhibitors are the key to a future of personalized medicine.
FDG-PET/CT is the standard imaging modality for staging of NSCLC and melanoma. Furthermore, tumor FDG-uptake is often used to assess response during therapy with ICI next to CT-based response criteria. Traditionally, response to therapy in NSCLC and melanoma is evaluated with the Response Evaluation Criteria in Solid Tumors (RECIST). However, responses in patients treated with ICI may show different response patterns compared to standard chemotherapy: Besides classic progression, stable disease and response to treatment, in some patients an initial increase of the tumor burden followed by a decrease as therapy continues can be seen, the so called pseudo-progression. To account for this phenomenon, several other standardized criteria such as the immune-related RECIST (irRECIST), the modified RECIST for immune-based therapeutics (iRECIST) and the PET response criteria in solid tumors (PERCIST) have been proposed to assess response to ICI. Clinical use, however, is difficult as no gold standard exist and measurements need to by repeated frequently, including an obligatory conformation after disease progression .
Since FDG-PET visualizes glucose metabolism, it can detect not only tumor cells, but also activated immune cells in and around the tumor, as well as in the rest of the body. As consequence, inflammatory side effects induced by ICI treatment, such as colitis (1), arthritis, Sjögren’s syndrome and thyroiditis, can be visualized by FDG-PET. Earlier studies have shown that the occurrence of ICI related side effects may be a positive predictor for a favorable outcome. Considering this, the presence of elevated FDG uptake in organs known to be susceptible for immune related side effects in patients treated with ICI may be an indicator of response.
In this retrospective, single center PET imaging analysis we want to assess whether the FDG uptake in different non-tumor tissues, as surrogate for the patients inflammatory state, is predictive for response to ICI. Furthermore, we will evaluate whether we can predict the occurrence of ICI related side effects by baseline FDG uptake of relevant tissues.
Research question / problem definition
Can we predict response to ICI treatment by measuring the body’s inflammatory state by means of FDG-PET?
Can FDG-PET aid as tool to identify immune-related adverse events in patients with metastatic melanoma or NSCLC receiving ICI treatment?
Can we use FDG-PET to distinguish between early, not-yet-clinically-relevant tissue inflammation, thus early AE, and never-clinically-relevant tissue inflammation?