Projectdetails

of


The differences in T-cells and its subpopulations in follicular fluid in patients with endometriosis.

Keywords:
IVF reproductive immunology endometriosis

Researchers:
Dr. A.E.P. Cantineau
J.R. Prins

Nature of the research:
Pilot study

Fields of study:
gynaecology

Background / introduction
Follicular fluid (FF) surrounds the granulosa cell-oocyte complex and is one of the mediating factors in the communication between the cells within the follicle. Literature reveals that human FF and its components are key factors to the success of natural fertilization. Among other substances, FF consists of multiple cytokines and immune cells, including interleukin 6 (IL6), IL12, sHLA-G, macrophages, NK cells, and lymphocytes. Together, these cells and cytokines might influence the oocyte-granulosa-cell complex. Altered balances of immune content might be involved in changes on folliculogenesis, oocyte maturation, oocyte quality, and ovulation. Furthermore, these altered balances are possibly involved in infertility associated with immune mediated diseases such as endometriosis.
Research question / problem definition
The aim of this research is to elaborate on the function and contents of FF and its immunological profile in patients with endometriosis compared to matched controls
Workplan
Prospective follicle fluid collection and processing. Flow cytometry to identify T cell subsets and regulatory T cells and Luminex cytokine profiling.
References
Lachapelle MH, Hemmings R, Roy DC, Falcone T & Miron P 1996 Flow cytometric evaluation of leukocyte subpopulations in the follicular fluids of infertile patients. Fertility and sterility 65 1135-1140.
Opøien HK, Fedorcsak P, Polec A, Stensen MH, Åbyholm T, Tanbo T 2013 Do endometriomas induce an inflammatory reaction in nearby follicles? Human Reproduction 28 1837-45.
Zhang T, De Carolis C, Man GCW & Wang CC 2018 The link between immunity, autoimmunity and endometriosis: a literature update. Autoimmunity reviews 17 945-955.
back to toptop

Laatst gewijzigd: 23 februari 2012